Ear Center: Digital Photography Using the OR Microscope

Camera Equipment

  1. Nikon D100 DSLR camera body (or similar body)
  2. 60mm F2.8 Micro Nikkor lens (This is a macro lens. The effective focal length is 90mm due to a 1.5x factor secondary to CCD size compared to 35mm film.)

Camera/Lens Settings

  1. Camera:
    1. Mode =Program
    2. ISO = 400
    3. White Balance = incandescent light
    4. File Size = medium jpeg (if you are using the images for PowerPoint slides)
    5. Lens : Set lens to "manual focus" (adjust the ring on the lens barrel) and adjust the focus to "infinity" on the distance scale


  1. Adjust camera body and lens settings as above
  2. Accurately focus the microscope on the field
  3. Hold the lens against the microscope's rubber cup on the ocular: compose the image & shoot
  4. check the image for content and focus

Post-Processing of the Image

Post-process in Photoshop CS4 or similar software program (Photoshop Elements, etc.) Perform the following in order:

  • Crop the image appropriately, then set image size to 150 pixels
  • Adjust Levels, Contrast, and Color
  • Adjust Brightness and Contrast
  • Save the image as a jpeg file in an appropriate folder
  • Always make backup copies